Erratum to: On-Line HPLC with Biochemical Detection for Screening Bioactive Compounds in Complex Matrixes

New Automated Screening System for the Determination of Basic Compounds in Urine by On-Line Extraction-HPLC- DAD

A new automated, qualitative screening HPLC method for the identification of basic compounds in urine has been established. A 1-ml volume of urine was automatedly extracted online and separated on two coupled strong cation-exchange columns under isocratic conditions. The use of the photodiode-array detector (DAD, λ=190-370 nm) gave access to a library of more than 2600 toxicologically relevant ...

Effect of Mobile Phase Additives on the Resolution of Four Bioactive Compounds by RP-HPLC

The use of mobile phase additives enhances the separation and resolution of the bioactive compounds on the C(18) column. Chlorogenic acid, caffeic acid, rutin, and scoparone from Herba Artemisiae Scopariae were investigated as the target compounds. Acetic acid, triethylamine, inorganic salts, and several ionic liquids were added as mobile phase additives into methanol/water (40:60, v/v). The re...

Development of On-Line High Performance Liquid Chromatography (HPLC)-Biochemical Detection Methods as Tools in the Identification of Bioactives

Biochemical detection (BCD) methods are commonly used to screen plant extracts for specific biological activities in batch assays. Traditionally, bioactives in the most active extracts were identified through time-consuming bio-assay guided fractionation until single active compounds could be isolated. Not only are isolation procedures often tedious, but they could also lead to artifact formati...

Computer-assisted separation and primary screening of bioactive compounds

on some bayesian statistical models in actuarial science with emphasis on claim count

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